Quantification of β-Sitosterol in Sesbania grandiflora Bark using High Performance Thin Layer Chromatography
Abstract
An efficient high performance thin layer chromatographic (HPTLC) method was optimized for the purpose of determining the levels of β-sitosterol (βS) in the bark of Sesbania grandiflora (S. grandiflora). The chromatographic separation was finished with a toluene: ethyl acetate: formic acid (50:15:5) mobile phase, and a densitometric scan was performed at 254 nm between 2 and 10 µg/mL. The developed strategy was linear, and the correlation coefficient was 0.9901. This study created and proved a straightforward HPTLC method that is precise, quick, selective, and simple for identifying and quantifying the phytochemical marker βS in S. grandiflora bark. The methanolic extract of S. grandiflora bark was determined to have a βS content of 153.09 µg/mg upon further investigation. The approach that was described above can be utilized to screen the presence of βS in herbal or pharmaceutical formulations, with potential applications in various industries.