Wetlands serve as a vital guarantee for regional and urban development. Therefore, it is of great significance to study the changes taken place in ecosystem services as well as their contributing factors during the collaborative conservation and management of water resources across varying regions. We have studies and coupled the remote sensing image data in the years of 2000, 2009 and 2019, so as to estimate the value of ecosystem services available. Furthermore, we have evaluated the biodiversity of the East Dongting Lake Area. The findings indicate that: (1) The area of the construction land in research area has experienced increases, whereas the area of arable land has witnessed constant declines. (2) The total value of ecosystem services available in research area decreased by RMB 1.53 billion. The value of ecosystem services provided by the arable land decreased by 745 million yuan. (3) The sensitivity of the coefficient of ecosystem service value of all types of land use to the ecosystem service value does not exceed 1, revealing the lack of elasticity of the value of ecosystem services provided by research area. (4) The species diversity index measured in the construction land is lower than other areas in research area. Judging from the analysis that the changes taken place in the land use will impose a significant impact on the service value of ecosystems and biodiversity, and the reasonable development and utilization will facilitate the improvement of the service value of ecosystems and biodiversity in East Dongting Lake Area.