Methodological Guide for Geobotanical Research on Rare, Endemic, and Medicinal Plants: A Case Study of the Ranunculaceae Family

Alibek Ydyrys1,2

Nashtay Mukhitdinov1

Anna Ivashchenko3

Zhadyra Ashirova1

Muratzhan Massimzhan1,2

Elmira Imаnova4

Meruyert Parmanbekova4

Murat Toktar5,6

Birlikaby Yeszhanov1

Marzhanay Ilesbek1, 2

Gulnaz Askerbay1,2,Email

Raushan Kaparbay1,2,Email

Faculty of biology and biotechnology, Al-Farabi Kazakh National University, Almaty, 050040, Kazakhstan
Biomedical Research Centre, Al-Farabi Kazakh National University, Al-Farabi Ave. 71, 050040, Kazakhstan
Department of Entomology, Institute of Zoology, Al-Farabi Ave. 93, Almaty, 050060, Kazakhstan
NPJSC «Kazakh women’s teacher training university», A05C9Y7, St. Gogol, 114 k1, Almaty, 050013, Kazakhstan
Department of Mine Surveying and Geodesy, Institute Mining and Metallurgical Institute named after O.A. Baikonurov, Satbayev University, Almaty, 050013, Kazakhstan
6 Department of Soil Ecology, Kazakh Research Institute of Soil Science and Agrochemistry named after U.U. Uspanov, Al-Farabi Ave. 75, Almaty, 050060, Kazakhstan

Abstract

Comprehensive studies of plant communities are essential for preserving rare and valuable species that are diminishing in number. Currently, there is an increasing demand for robust methodologies to conduct population-level research in geobotany, ecological monitoring, and forestry. This article focuses on the rare plant species Hepatica falconeri, Ranunculus polyanthemus L., Ranunculus oxyspermus, and Ranunculus dilatatus, which belong to the Ranunculaceae family and are found in the Tien Shan Mountains of Kazakhstan, based on geobotanical research findings. We present a manual that integrates both traditional and modern techniques for studying environmental factors, biotopes, ectopes, phytocenoses, floral composition, and the condition of natural populations of endemic and medicinal plants. This includes methodologies for assessing the biomass reserves of rare, endemic, and medicinal species, as well as evaluating the state of juvenile plants, population density, and the anatomical, morphological, phytochemical, and molecular genetic characteristics of the studied taxa. These parameters are influenced by geographical variations, microclimatic conditions, and soil properties. This article serves as a comprehensive guide for researchers in the fields of geobotany, biodiversity, botany, biology, agriculture, and medicinal plant studies. Additionally, it provides actionable recommendations for developing effective strategies for the conservation, protection, and sustainable utilization of biodiversity.