The pollution of terrestrial soils with petroleum and its derivatives presents considerable ecological challenges, thus requiring the development of efficacious remediation methodologies. This analysis investigates the application of humic substances and microorganisms in the rehabilitation of soils contaminated by oil. Humic substances, recognized for their capacity to improve soil structure and fertility, function as natural chelators, facilitating the degradation and extraction of hydrocarbons. Microorganisms, especially those possessing hydrocarbon-degrading attributes, are integral to the bioremediation process. The synergistic utilization of these biological agents expedites the breakdown of oil contaminants, thereby enhancing soil vitality and productivity. Empirical field trials and controlled laboratory investigations reveal that the combined application of humic substances and microorganisms markedly diminishes hydrocarbon concentrations in polluted soils. This methodology not only alleviates the ecological repercussions of oil contamination but also fosters sustainable land management and ecosystem restoration. The results emphasize the promise of amalgamating natural substances and microbial processes within effective soil rehabilitation strategies.