This work explores the intricate relationship between biodiesel and engine vibration, a critical factor in assessing diesel engine performance with alternative fuels. Focusing on combustion characteristics, fuel qualities, and influential factors, the study examines how Biodiesel alters engine vibration, emphasizing connections with combustion dynamics. The investigation delves into biodiesel characteristics like viscosity and volatility, exploring their impact on atomization and combustion-induced vibration patterns. Factors such as lower heating value, oxygen content, injection timing, and bulk modulus are also considered. The review aims to consolidate existing research, offering a comprehensive resource for academics and stakeholders in sustainable engine technologies. It discusses Biodiesel's potential benefits and challenges, contributing to a nuanced understanding of its multidimensional impact on engine vibrations. This study enhances knowledge of Biodiesel's role in sustainable fuel usage within internal combustion engine vibrations.