This investigation examined the influence of iron oxide nanoparticles (IONP) on the anaerobic co-digestion of chicken manure (CM) and sewage sludge (SS). The experiment was conducted in many batches, with the temperature maintained at 35°C, using a RESPIROMETRIC Sensor System 6 Maxi—BMP (RSS-BMP). The utilisation of IONP at dosages of 40, 80, 120, and 160 mg/L in batches M1, M2, M3, and M4. The results showed that the introduction of IONP at a concentration of 40 mg/L into anaerobic batches had no significant effect on the methanogenesis process and the consequent generation of methane. Exposing the samples to IONP at doses of 80, 120, and 160 mg/L had a comparable beneficial impact. In addition, there was also an increase in methane production. The concentration of CH4 was increased by 32.5%, 50.5%, and 62.7% when employing 80, 120, and 160 mg/L IONP, respectively, compared to the control test. IONP, when present at a concentration of 160 mg/L, achieved a biodegradability of 96.7%, but the control incubation (C4) only achieved a biodegradability of 56.1%.