This review is providing a comprehensive overview of essential genuine characterization techniques for nanomaterial and biomaterials exist in various forms. Nanoscience and nanotechnology is one of the Trans scientific frontier, multidisciplinary and environmentally sustainable research field. Today nanomaterials are widely employing in almost every branches of science and technology. As nanomaterials are invisible and unknown in size, shape, so it is enormously needy the advanced characterizations tools to visualize and analyze the materials at nanoscale. The characterization techniques are of paramount importance in the field of nanoscience and technology. This review is to summarize the present knowledge on the use, advances, advantages and weaknesses of a large number of experimental techniques that are available for the characterization of nanoparticles. Different characterization techniques are classified according to the concept/group of the technique used, the information they can provide, or the materials that they are destined for. Many times the beginner researcher in the domain is not aware with the advanced characterization techniques and its purposes for data interpretation.